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collagen coated glass controls  (Thermo Fisher)


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    Structured Review

    Thermo Fisher collagen coated glass controls
    Growth of metastatic KM12L4a cells on top of collagen functionalized PAAm hydrogels, <t>and</t> <t>collagen-coated</t> glass (control). Images are representative of cells before proteomic analysis: after 24 h cell growth, 48 h starvation, and three washes with D-PBS. A , representative transmission images. These scale bars represents 200 μm and 100 μm, for full size and magnifications, respectively. B , brightfield quantification of cell aggregate morphology (density, area, perimeter). Data is represented in a violin plot where solid lines show medians, while dashed lines show quartiles. Data was obtained from minimally 2 fields of view, from three biological replicates. C , representative 3D view of tubulin ( cyan ) and 6-Diamidino-2-Phenylindole ( magenta ) in KM12L4a cells grown on 2 and 47 kPa hydrogels. Grid represents 3D-view, with XY scale bar represents: 50 μm. D , 3D morphological quantification for different parameters. Data was obtained from n = 30 samples, from three biological replicates. B and D , significance levels: (−) p > 0.05, non-significant; (∗∗∗∗) p ≤ 0.0001.
    Collagen Coated Glass Controls, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+coated+glass+controls/Collagen/pmc12964026-42-0-35
    Average 99 stars, based on 1 article reviews
    collagen coated glass controls - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics"

    Article Title: Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1016/j.mcpro.2026.101515

    Growth of metastatic KM12L4a cells on top of collagen functionalized PAAm hydrogels, and collagen-coated glass (control). Images are representative of cells before proteomic analysis: after 24 h cell growth, 48 h starvation, and three washes with D-PBS. A , representative transmission images. These scale bars represents 200 μm and 100 μm, for full size and magnifications, respectively. B , brightfield quantification of cell aggregate morphology (density, area, perimeter). Data is represented in a violin plot where solid lines show medians, while dashed lines show quartiles. Data was obtained from minimally 2 fields of view, from three biological replicates. C , representative 3D view of tubulin ( cyan ) and 6-Diamidino-2-Phenylindole ( magenta ) in KM12L4a cells grown on 2 and 47 kPa hydrogels. Grid represents 3D-view, with XY scale bar represents: 50 μm. D , 3D morphological quantification for different parameters. Data was obtained from n = 30 samples, from three biological replicates. B and D , significance levels: (−) p > 0.05, non-significant; (∗∗∗∗) p ≤ 0.0001.
    Figure Legend Snippet: Growth of metastatic KM12L4a cells on top of collagen functionalized PAAm hydrogels, and collagen-coated glass (control). Images are representative of cells before proteomic analysis: after 24 h cell growth, 48 h starvation, and three washes with D-PBS. A , representative transmission images. These scale bars represents 200 μm and 100 μm, for full size and magnifications, respectively. B , brightfield quantification of cell aggregate morphology (density, area, perimeter). Data is represented in a violin plot where solid lines show medians, while dashed lines show quartiles. Data was obtained from minimally 2 fields of view, from three biological replicates. C , representative 3D view of tubulin ( cyan ) and 6-Diamidino-2-Phenylindole ( magenta ) in KM12L4a cells grown on 2 and 47 kPa hydrogels. Grid represents 3D-view, with XY scale bar represents: 50 μm. D , 3D morphological quantification for different parameters. Data was obtained from n = 30 samples, from three biological replicates. B and D , significance levels: (−) p > 0.05, non-significant; (∗∗∗∗) p ≤ 0.0001.

    Techniques Used: Control, Transmission Assay

    Related Articles

    Sterility:

    Article Title: Deciphering stiffness-driven changes in colorectal cancer by proteomics.
    Article Snippet: After washing with 10 mL sterile dH2O (3x), the gel was incubated overnight with 165 μg collagen type I (Thermo Fisher Scientific, A1048301) diluted in 10 mL sterile 0.5% acetic acid (Acros Organics, 64-19-7, 99.8%, pH 3.40) to ensure homogeneous coating (25 μg/cm2 coating density).38 The coated gel was then washed with 10 mL sterile DPBS (3x) and equilibrated in 10 mL Jo urn al P e-p roo f 5 prewarmed sterile cell culture medium at 37 °C and 5% CO2 for at least 30 minutes before cell seeding. .. Collagen-coated glass controls were prepared in a glass-bottom 6-well plate with wells of 35 mm diameter (Cellvis, P06-1.5H-N), by washing with dH2O (3x), incubating overnight with 23.9 μg collagen type I per well (Thermo Fisher Scientific, A1048301) diluted in 1.45 mL sterile 0.5% acetic acid (Acros Organics, 64-19-7, 99.8%, pH 3.40) to ensure the same coating density as on the PAAm gels. ..

    Article Title: Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics
    Article Snippet: .. Collagen-coated glass controls were prepared in a glass-bottom 6-well plate with wells of 35 mm diameter (Cellvis, P06–1.5H-N), by washing with dH 2 O (3×), incubating overnight with 23.9 μg collagen type I per well (Thermo Fisher Scientific, A1048301) diluted in 1.45 ml sterile 0.5% acetic acid (Acros Organics, 64–19–7, 99.8%, pH 3.4) to ensure the same coating density as on the PAAm gels. ..



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    Growth of metastatic KM12L4a cells on top of collagen functionalized PAAm hydrogels, <t>and</t> <t>collagen-coated</t> glass (control). Images are representative of cells before proteomic analysis: after 24 h cell growth, 48 h starvation, and three washes with D-PBS. A , representative transmission images. These scale bars represents 200 μm and 100 μm, for full size and magnifications, respectively. B , brightfield quantification of cell aggregate morphology (density, area, perimeter). Data is represented in a violin plot where solid lines show medians, while dashed lines show quartiles. Data was obtained from minimally 2 fields of view, from three biological replicates. C , representative 3D view of tubulin ( cyan ) and 6-Diamidino-2-Phenylindole ( magenta ) in KM12L4a cells grown on 2 and 47 kPa hydrogels. Grid represents 3D-view, with XY scale bar represents: 50 μm. D , 3D morphological quantification for different parameters. Data was obtained from n = 30 samples, from three biological replicates. B and D , significance levels: (−) p > 0.05, non-significant; (∗∗∗∗) p ≤ 0.0001.
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    Growth of metastatic KM12L4a cells on top of collagen functionalized PAAm hydrogels, <t>and</t> <t>collagen-coated</t> glass (control). Images are representative of cells before proteomic analysis: after 24 h cell growth, 48 h starvation, and three washes with D-PBS. A , representative transmission images. These scale bars represents 200 μm and 100 μm, for full size and magnifications, respectively. B , brightfield quantification of cell aggregate morphology (density, area, perimeter). Data is represented in a violin plot where solid lines show medians, while dashed lines show quartiles. Data was obtained from minimally 2 fields of view, from three biological replicates. C , representative 3D view of tubulin ( cyan ) and 6-Diamidino-2-Phenylindole ( magenta ) in KM12L4a cells grown on 2 and 47 kPa hydrogels. Grid represents 3D-view, with XY scale bar represents: 50 μm. D , 3D morphological quantification for different parameters. Data was obtained from n = 30 samples, from three biological replicates. B and D , significance levels: (−) p > 0.05, non-significant; (∗∗∗∗) p ≤ 0.0001.
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    Image Search Results


    Growth of metastatic KM12L4a cells on top of collagen functionalized PAAm hydrogels, and collagen-coated glass (control). Images are representative of cells before proteomic analysis: after 24 h cell growth, 48 h starvation, and three washes with D-PBS. A , representative transmission images. These scale bars represents 200 μm and 100 μm, for full size and magnifications, respectively. B , brightfield quantification of cell aggregate morphology (density, area, perimeter). Data is represented in a violin plot where solid lines show medians, while dashed lines show quartiles. Data was obtained from minimally 2 fields of view, from three biological replicates. C , representative 3D view of tubulin ( cyan ) and 6-Diamidino-2-Phenylindole ( magenta ) in KM12L4a cells grown on 2 and 47 kPa hydrogels. Grid represents 3D-view, with XY scale bar represents: 50 μm. D , 3D morphological quantification for different parameters. Data was obtained from n = 30 samples, from three biological replicates. B and D , significance levels: (−) p > 0.05, non-significant; (∗∗∗∗) p ≤ 0.0001.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics

    doi: 10.1016/j.mcpro.2026.101515

    Figure Lengend Snippet: Growth of metastatic KM12L4a cells on top of collagen functionalized PAAm hydrogels, and collagen-coated glass (control). Images are representative of cells before proteomic analysis: after 24 h cell growth, 48 h starvation, and three washes with D-PBS. A , representative transmission images. These scale bars represents 200 μm and 100 μm, for full size and magnifications, respectively. B , brightfield quantification of cell aggregate morphology (density, area, perimeter). Data is represented in a violin plot where solid lines show medians, while dashed lines show quartiles. Data was obtained from minimally 2 fields of view, from three biological replicates. C , representative 3D view of tubulin ( cyan ) and 6-Diamidino-2-Phenylindole ( magenta ) in KM12L4a cells grown on 2 and 47 kPa hydrogels. Grid represents 3D-view, with XY scale bar represents: 50 μm. D , 3D morphological quantification for different parameters. Data was obtained from n = 30 samples, from three biological replicates. B and D , significance levels: (−) p > 0.05, non-significant; (∗∗∗∗) p ≤ 0.0001.

    Article Snippet: Collagen-coated glass controls were prepared in a glass-bottom 6-well plate with wells of 35 mm diameter (Cellvis, P06–1.5H-N), by washing with dH 2 O (3×), incubating overnight with 23.9 μg collagen type I per well (Thermo Fisher Scientific, A1048301) diluted in 1.45 ml sterile 0.5% acetic acid (Acros Organics, 64–19–7, 99.8%, pH 3.4) to ensure the same coating density as on the PAAm gels.

    Techniques: Control, Transmission Assay